| Nature 2013 Idris et al | However I don't understand the chemistry at the molecular level and am hoping someone more knowledgeable can help |
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| However, our company INTCO has several machines which can deal with the problem | PNAS 2012 Aldridge and Hadfield |
Smaller fragments are retained in the buffer usually discarded and so you can get different size ranges from a single sample with multiple purifications.
| Merci Bonjour Corinne, Quel plaisir ton site! Side note, smaller nucleic acid polymers are more soluble in these alcohol solutions due to their smaller size - form smaller condensed balls | Molecular Cell 2008 Smith et al |
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| The final DNA product is eluted from beads for further processing e | The size of fragments eluted from the beads or that bind in the first place is determined by the concentration of PEG, and this in turn is determined by the mix of DNA and beads |
Substituting size-fractionated silica particles for diatoms the fossilized cell walls of unicellular algae allowed for the purification of microgram amounts of genomic DNA, plasmid DNA, and rRNA from cell-rich sources, as exemplified for pathogenic gram-negative bacteria.